miR-203 suppresses tumor growth and angiogenesis by targeting VEGFA in cervical cancer.

نویسندگان

  • Xiangyu Zhu
  • Kejun Er
  • Caiying Mao
  • Qi Yan
  • Haijun Xu
  • Yuanbei Zhang
  • Jianhong Zhu
  • Fang Cui
  • Wenxia Zhao
  • Hong Shi
چکیده

BACKGROUND/AIMS MicroRNA (miRNA) plays important roles in the development of different cancers. In this study, we investigated the roles and mechanisms of miR-203 in human cervical cancer. METHODS miR-203 expression was detected in cervical cancer tumors and cell lines by qRT-PCR. The methylation status in the promoter region of miR-203 was examined by methylation-specific PCR. The functional effect of miR-203 was determined by both in vitro and in vivo assays. RESULTS miR-203 was frequently down-regulated in cervical cancer tumors and cell lines. This down-regulation of miR-203 was associated with methylation of the miR-203 promoter. Furthermore, miR-203 down-regulated vascular endothelial growth factor alpha (VEGFA) expression by directly targeting its 3'-untranslated region. Functional assays revealed that miR-203 suppressed cervical cancer cell proliferation, tumor growth, and angiogenesis in nude mice, whereas forced expression of VEGFA rescued this inhibitory effect. CONCLUSION Our collective findings indicate that miR-203 functions as a tumor suppressor by targeting VEGFA, resulting in the inhibition of tumor growth and angiogenesis. Thus, miR-203 may be a potential therapeutic target and prognostic marker in cervical cancer.

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عنوان ژورنال:
  • Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

دوره 32 1  شماره 

صفحات  -

تاریخ انتشار 2013